Experimental detection of knotted conformations in denatured proteins.

  title={Experimental detection of knotted conformations in denatured proteins.},
  author={Anna L. Mallam and Joseph M. Rogers and Sophie E. Jackson},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={107 18},
Structures that contain a knot formed by the path of the polypeptide backbone represent some of the most complex topologies observed in proteins. How or why these topological knots arise remains unclear. By developing a method to experimentally trap and detect knots in nonnative polypeptide chains, we find that two knotted methyltransferases, YibK and YbeA, can exist in a trefoil-knot conformation even in their chemically unfolded states. The unique denatured-state topology of these molecules… CONTINUE READING
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