Experimental design in the development and characterization of a high-performance liquid chromatographic method for amino acids

@inproceedings{Hansen1992ExperimentalDI,
  title={Experimental design in the development and characterization of a high-performance liquid chromatographic method for amino acids},
  author={H. A. Hansen and Claus Emborg},
  year={1992}
}
In the practical establishment of an HPLC method, it is often necessary to do a considerable amount of experimentation even with methods that are not new. In this paper it is demonstrated how the structuring of a considerable part of the experiments in two-level factorial designs facilitates the localization of useful chromatographic conditions, while at the same time the system is characterized with respect to factors and interactions between factors that have an influence on the quality of… CONTINUE READING