Experimental and in silico analyses of glycolytic flux control in bloodstream form Trypanosoma brucei.

@article{Albert2005ExperimentalAI,
  title={Experimental and in silico analyses of glycolytic flux control in bloodstream form Trypanosoma brucei.},
  author={Marie-Astrid Albert and Jurgen R Haanstra and V{\'e}ronique Hannaert and Joris Van Roy and Fred R. Opperdoes and Barbara M. Bakker and Paul A. M. Michels},
  journal={The Journal of biological chemistry},
  year={2005},
  volume={280 31},
  pages={28306-15}
}
A mathematical model of glycolysis in bloodstream form Trypanosoma brucei was developed previously on the basis of all available enzyme kinetic data (Bakker, B. M., Michels, P. A. M., Opperdoes, F. R., and Westerhoff, H. V. (1997) J. Biol. Chem. 272, 3207-3215). The model predicted correctly the fluxes and cellular metabolite concentrations as measured in non-growing trypanosomes and the major contribution to the flux control exerted by the plasma membrane glucose transporter. Surprisingly, a… CONTINUE READING