Experimental Liver Injury Induced by Propionibacterium acnes and Lipopolysaccharide in Macrophage Colony Stimulating Factor-Deficient Osteopetrotic (op/op) Mice

Abstract

To clarify the involvement of growth anddifferention of liver macrophages mediated by macrophagecolony-stimulating factor (M-CSF) in the liver injuryinduced by Propionibacterium acnes (P. acnes) and lipopolysaccharide (LPS), we usedM-CSF-deficient osteopetrotic (op/op) mice. Seven daysafter injection of P. acnes, granulomas as well as thenumbers of Thy-1.2-, Mac-1-, and ERMP-20-positive cellsand F4/80-positive areas in the liver weresignificantly reduced in the op/op mice compared to thenormal littermates. After injection of LPS, serum levelsof alanine aminotransferase as well as concentrations of IL-1β and TNF-α in the serum andliver were significantly lower in the op/op mice than inthe normal littermates, whereas the concentrations ofIL-1β and TNF-α in the spleen were similar in op/op mice and normal littermates. Theseresults suggest that M-CSF plays a partial but highlysignificant role in the development of liver injuryinduced by P. acnes and LPS via an intrahepatic increase of primed macrophages including those ingranulomas, in response to P. acnes, which produceproinflammatory cytokines such as IL-1β andTNF-α.

DOI: 10.1023/A:1026653830920

11 Figures and Tables

Statistics

0100200'01'03'05'07'09'11'13'15'17
Citations per Year

271 Citations

Semantic Scholar estimates that this publication has 271 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Nishioji1999ExperimentalLI, title={Experimental Liver Injury Induced by Propionibacterium acnes and Lipopolysaccharide in Macrophage Colony Stimulating Factor-Deficient Osteopetrotic (op/op) Mice}, author={Kenichi Nishioji and Takeshi Okanoue and Takashi Mori and Shinichi Sakamoto and Yoshito Itoh}, journal={Digestive Diseases and Sciences}, year={1999}, volume={44}, pages={1975-1984} }