Expansion on Stromal Cells Preserves the Undifferentiated State of Human Hematopoietic Stem Cells Despite Compromised Reconstitution Ability

@inproceedings{Magnusson2013ExpansionOS,
  title={Expansion on Stromal Cells Preserves the Undifferentiated State of Human Hematopoietic Stem Cells Despite Compromised Reconstitution Ability},
  author={Mattias Magnusson and Maria I. Sierra and Rajkumar Sasidharan and Sacha Leandra Prashad and Melissa M Romero and Pamela Saarikoski and Ben van Handel and Andy Huang and Xinmin Li and Hanna K. A. Mikkola},
  booktitle={PloS one},
  year={2013}
}
Lack of HLA-matched hematopoietic stem cells (HSC) limits the number of patients with life-threatening blood disorders that can be treated by HSC transplantation. So far, insufficient understanding of the regulatory mechanisms governing human HSC has precluded the development of effective protocols for culturing HSC for therapeutic use and molecular studies. We defined a culture system using OP9M2 mesenchymal stem cell (MSC) stroma that protects human hematopoietic stem/progenitor cells (HSPC… CONTINUE READING