Expansion of Human Airway Basal Stem Cells and Their Differentiation as 3D Tracheospheres.

Abstract

Although basal cells function as human airway epithelial stem cells, analysis of these cells is limited by in vitro culture techniques that permit only minimal cell growth and differentiation. Here, we report a protocol that dramatically increases the long-term expansion of primary human airway basal cells while maintaining their genomic stability using 3T3… (More)

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