Expansion microscopy

  title={Expansion microscopy},
  author={Fei Chen and Paul W. Tillberg and Edward S. Boyden},
In optical microscopy, fine structural details are resolved by using refraction to magnify images of a specimen. We discovered that by synthesizing a swellable polymer network within a specimen, it can be physically expanded, resulting in physical magnification. By covalently anchoring specific labels located within the specimen directly to the polymer network, labels spaced closer than the optical diffraction limit can be isotropically separated and optically resolved, a process we call… CONTINUE READING