Expanding substrate specificity of nucleoside 2'-deoxyribosyltransferase.

Abstract

Nucleoside 2'-deoxyribosyltransferase (NDT) is used to synthesize unnatural 2'-deoxyribonucleosides, modified mostly on the heterocyclic base. Here we describe a strategy for improving 2,3-dideoxyribosyl(ddR) transfer activity of NDT by combining mutagenesis and in vivo selection in E. coli.

DOI: 10.1093/nass/nrn251

Cite this paper

@article{Kaminski2008ExpandingSS, title={Expanding substrate specificity of nucleoside 2'-deoxyribosyltransferase.}, author={Pierre Alexandre Kaminski and Laurence Dugu{\'e} and Sylvie Pochet}, journal={Nucleic acids symposium series}, year={2008}, volume={52}, pages={495-6} }