Expanding pyrimidine diphosphosugar libraries via structure-based nucleotidylyltransferase engineering.

  title={Expanding pyrimidine diphosphosugar libraries via structure-based nucleotidylyltransferase engineering.},
  author={William A Barton and John B. Biggins and Jiqing Jiang and Jon S Thorson and Dimitar B. Nikolov},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={99 21},
In vitro "glycorandomization" is a chemoenzymatic approach for generating diverse libraries of glycosylated biomolecules based on natural product scaffolds. This technology makes use of engineered variants of specific enzymes affecting metabolite glycosylation, particularly nucleotidylyltransferases and glycosyltransferases. To expand the repertoire of UDP/dTDP sugars readily available for glycorandomization, we now report a structure-based engineering approach to increase the diversity of… CONTINUE READING
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