Expanded instrument comparison of amplicon DNA melting analysis for mutation scanning and genotyping.

@article{Herrmann2007ExpandedIC,
  title={Expanded instrument comparison of amplicon DNA melting analysis for mutation scanning and genotyping.},
  author={Mark G. Herrmann and Jacob Durtschi and Carl T. Wittwer and Karl V. Voelkerding},
  journal={Clinical chemistry},
  year={2007},
  volume={53 8},
  pages={1544-8}
}
BACKGROUND Additional instruments have become available since instruments for DNA melting analysis of PCR products for genotyping and mutation scanning were compared. We assessed the performance of these new instruments for genotyping and scanning for mutations. METHODS A 110-bp fragment of the beta-globin gene including the sickle cell anemia locus (HBB c. 20A>T) was amplified by PCR in the presence of LCGreen Plus or SYBR Green I. Amplicons of 4 different genotypes [wild-type, homozygous… CONTINUE READING

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