Expanded-bed chromatography in primary protein purification.

@article{Anspach1999ExpandedbedCI,
  title={Expanded-bed chromatography in primary protein purification.},
  author={F. Birger Anspach and David Curbelo and Romuald Hartmann and Gunnar Garke and W. D. Deckwer},
  journal={Journal of chromatography. A},
  year={1999},
  volume={865 1-2},
  pages={
          129-44
        }
}
Chromatography in stable expanded beds enables proteins to be recovered directly from cultivations of microorganisms or cells and preparations of disrupted cells, without the need for prior removal of suspended solids. The general performance of an expanded bed is comparable to a packed bed owing to reduced mixing of the adsorbent particles in the column. However, optimal operating conditions are more restricted than in a packed bed due to the dependence of bed expansion on the size and density… CONTINUE READING
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