Expanded RNA-binding activities of mammalian Argonaute 2

  title={Expanded RNA-binding activities of mammalian Argonaute 2},
  author={Grace S. Tan and Barry G. Garchow and Xuhang Liu and Jennifer Yeung and John P. Morris and Trinna L Cuellar and Michael T. McManus and Marianthi Kiriakidou},
  booktitle={Nucleic acids research},
Mammalian Argonaute 2 (Ago2) protein associates with microRNAs (miRNAs) or small interfering RNAs (siRNAs) forming RNA-induced silencing complexes (RISCs/miRNPs). In the present work, we characterize the RNA-binding and nucleolytic activity of recombinant mouse Ago2. Our studies show that recombinant mouse Ago2 binds efficiently to miRNAs forming active RISC. Surprisingly, we find that recombinant mouse Ago2 forms active RISC using pre-miRNAs or long unstructured single stranded RNAs as guides… CONTINUE READING
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