Exosome-mediated delivery of siRNA in vitro and in vivo

@article{ElAndaloussi2012ExosomemediatedDO,
  title={Exosome-mediated delivery of siRNA in vitro and in vivo},
  author={Samir El-Andaloussi and Yi Xin Fiona Lee and Samira Lakhal-Littleton and Jinghuan Li and Yiqi Seow and Chris Gardiner and Lydia Alvarez-Erviti and Ian L. Sargent and Matthew J. A. Wood},
  journal={Nature Protocols},
  year={2012},
  volume={7},
  pages={2112-2126}
}
The use of small interfering RNAs (siRNAs) to induce gene silencing has opened a new avenue in drug discovery. However, their therapeutic potential is hampered by inadequate tissue-specific delivery. Exosomes are promising tools for drug delivery across different biological barriers. Here we show how exosomes derived from cultured cells can be harnessed for delivery of siRNA in vitro and in vivo. This protocol first describes the generation of targeted exosomes through transfection of an… 
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