Exosome-mediated delivery of siRNA in vitro and in vivo

@article{ElAndaloussi2012ExosomemediatedDO,
  title={Exosome-mediated delivery of siRNA in vitro and in vivo},
  author={S. El‐Andaloussi and Y. Lee and S. Lakhal-Littleton and J. Li and Y. Seow and C. Gardiner and L. Alvarez-Erviti and I. Sargent and M. Wood},
  journal={Nature Protocols},
  year={2012},
  volume={7},
  pages={2112-2126}
}
  • S. El‐Andaloussi, Y. Lee, +6 authors M. Wood
  • Published 2012
  • Medicine, Biology
  • Nature Protocols
  • The use of small interfering RNAs (siRNAs) to induce gene silencing has opened a new avenue in drug discovery. However, their therapeutic potential is hampered by inadequate tissue-specific delivery. Exosomes are promising tools for drug delivery across different biological barriers. Here we show how exosomes derived from cultured cells can be harnessed for delivery of siRNA in vitro and in vivo. This protocol first describes the generation of targeted exosomes through transfection of an… CONTINUE READING

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