Exonuclease VII of Escherichia coli.

@article{Chase1975ExonucleaseVO,
  title={Exonuclease VII of Escherichia coli.},
  author={J. W. Chase and Charles C. Richardson},
  journal={Basic life sciences},
  year={1975},
  volume={5A},
  pages={225-34}
}
A new exonuclease of Escherichia coli K12, exonuclease VII, has been purified 1700-fold and characterized. The enzyme is specific for single-stranded DNA and can initiated hydrolysis at both 5' and 3' termini. It is also capable of thymine-dimer excision in vitro. The limit products of the reaction are oligonucleotides, predominantly in the range of tetramers to dodecamers. DNA is hydrolyzed by the enzyme in a processive fashion. Mutants of E. coli have been isolated having reduced levels of… CONTINUE READING

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