Exonuclease III footprinting on immobilized DNA templates.

Abstract

DNA footprinting is a widely used method to locate the binding sites of protein on the DNA. It is based on the observation that a protein bound to DNA protects it from degradation by an enzyme or chemical reagent.Exonuclease III is a suitable probe to analyze the boundaries of a protein when it is necessary to eliminate any excess unbound DNA from the… (More)
DOI: 10.1007/978-1-60327-015-1_4

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