Exogenous polyunsaturated fatty acids (PUFAs) alter phospholipid composition, membrane permeability, biofilm formation and motility in Acinetobacter baumannii.

@article{Eder2017ExogenousPF,
  title={Exogenous polyunsaturated fatty acids (PUFAs) alter phospholipid composition, membrane permeability, biofilm formation and motility in Acinetobacter baumannii.},
  author={Adrianna E Eder and Saba Munir and Chelsea R Hobby and Derek M Anderson and Joshua L Herndon and Andrew W Siv and Steven J. K. Symes and David K Giles},
  journal={Microbiology},
  year={2017},
  volume={163 11},
  pages={
          1626-1636
        }
}
Acinetobacter baumannii is a ubiquitous multidrug-resistant bacteria that is found on a variety of surfaces, including skin, hair and soil. During the past decade, A. baumannii has emerged as a significant cause of nosocomial infections in the United States. Recent studies have highlighted the ability of some bacteria to utilize a wide variety of fatty acids as a membrane remodelling strategy. Considering this, we hypothesized that fatty acids may have an effect on the emerging pathogen A… 

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References

SHOWING 1-10 OF 41 REFERENCES
Remodelling of the Vibrio cholerae membrane by incorporation of exogenous fatty acids from host and aquatic environments
TLDR
The results suggest that Vibrio species possess unique machinery conferring the ability to take up a wider range of exogenous fatty acids than other enteric bacteria.
Multiple FadD Acyl-CoA Synthetases Contribute to Differential Fatty Acid Degradation and Virulence in Pseudomonas aeruginosa
TLDR
Ch Chromosomal mutagenesis, growth analysis, induction studies, and determination of kinetic parameters suggested that FadD1 has a substrate preference for long-chain FAs while fadD2 prefers shorter- Chain FAs, supporting the role of lipids as a significant nutrient source for this bacterium in vivo.
A Fatty Acid Messenger Is Responsible for Inducing Dispersion in Microbial Biofilms
TLDR
It is demonstrated that, during growth, Pseudomonas aeruginosa produces an organic compound, identified as cis-2-decenoic acid, which is capable of inducing the dispersion of established biofilms and of inhibiting biofilm development.
Pseudomonas aeruginosa Directly Shunts β-Oxidation Degradation Intermediates into De Novo Fatty Acid Biosynthesis
TLDR
It is shown that growth defects stemming from deletion of fabY can be suppressed by supplementation of the growth media with exogenous decanoate fatty acid, suggesting a compensatory mechanism.
Multi-omics approach to study global changes in a triclosan-resistant mutant strain of Acinetobacter baumannii ATCC 17978.
Genetic analysis of surface motility in Acinetobacter baumannii.
TLDR
Transposon mutagenesis was used to identify additional genes required for motility and revealed loci encoding various functions: non-ribosomal synthesis of a putative lipopeptide, a sensor kinase (BfmS), a lytic transglycosylase, O-antigen biosynthesis, an outer membrane porin (OmpA) and de novo purine biosynthesis (PurK).
Exogenous fatty acid metabolism in bacteria.
Do Biofilm Formation and Interactions with Human Cells Explain the Clinical Success of Acinetobacter baumannii?
TLDR
The induction of a weak inflammatory response may provide a clue to the persistence of A. baumannii in patients as well as other, clinically less relevant Acinetobacter species.
H-NS Plays a Role in Expression of Acinetobacter baumannii Virulence Features
TLDR
Analysis of virulence features of a hypermotile derivative of A. baumannii strain ATCC 17978 revealed modifications to the fatty acid composition, providing a possible explanation for the observed changes in hydrophobicity and subsequent alteration in adherence and motility.
...
1
2
3
4
5
...