Exogenous glutathione is essential in the testing of antioxidant capacity using radical-induced haemolysis.

INTRODUCTION Radical-induced haemolysis has been employed by many investigators to determine the antioxidant capacity of novel compounds. However the free radical generator 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH) results in the complete depletion of intracellular reduced glutathione (GSH) in cells that can no longer synthesise macromolecules… CONTINUE READING