Exogenous gene expression and protein targeting in lens fiber cells.

Abstract

PURPOSE To test the ability of lens fiber cells at various stages of differentiation to transcribe and translate microinjected DNA templates. METHODS Expression plasmids encoding green fluorescent protein (GFP) or a GFP-tagged membrane protein (human CD46) were microinjected into organ-cultured embryonic chicken lenses. Protein expression was visualized… (More)

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