Exogenous fructose-1,6-bisphosphate is a metabolizable substrate for the isolated normoxic rat heart

@article{Tavazzi1992ExogenousFI,
  title={Exogenous fructose-1,6-bisphosphate is a metabolizable substrate for the isolated normoxic rat heart},
  author={Barbara Tavazzi and Joseph W. Starnes and Giuseppe Lazzarino and Donato Di Pierro and E. Matti Nuutinen and Bruno Giardina},
  journal={Basic Research in Cardiology},
  year={1992},
  volume={87},
  pages={280-289}
}
Isolated rat hearts were perfused by the recirculating Langendorff mode under normoxic conditions for 60 min. The Krebs-Ringer buffer was supplemented with 10mM glucose+12 IU/l insulin and either [U-14C]-fructose-1,6-bisphosphate (together with 5 mM cold fructose-1,6-bisphosphate) or [U-14C]-fructose (together with 5 mM cold fructose). At the end of perfusion, gaseous14CO2,14CO2 trapped in the perfusates,14C-lactate output and tissue14C-lactate were assayed in both groups of hearts. Analysis of… CONTINUE READING

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