Exogenous Nucleation Sites Fail to Induce Detectable Polymerization of Actin in Living Cells

@inproceedings{Sander2002ExogenousNS,
  title={Exogenous Nucleation Sites Fail to Induce Detectable Polymerization of Actin in Living Cells},
  author={Mitchel l C. Sander},
  year={2002}
}
Most nonmuscle cells are known to maintain a relatively high concentration of unpolymerized actin. To determine how the polymerization of actin is regulated, exogenous nucleation sites, prepared by sonicating fluorescein phalloidin-labeled actin filaments, were microinjected into living Swiss 3T3 and NRK cells. The nucleation sites remained as a cluster for over an hour after microinjection, and caused no detectable change in the phase morphology of the cell. As determined by immunofluorescence… CONTINUE READING