Exhaustive analysis of the P53 gene coding sequence by denaturing gradient gel electrophoresis: application to the detection of point mutations in acute leukemias.

@article{Pignon1994ExhaustiveAO,
  title={Exhaustive analysis of the P53 gene coding sequence by denaturing gradient gel electrophoresis: application to the detection of point mutations in acute leukemias.},
  author={J M Pignon and I Vinatier and Pascale Fanen and Philippe Jonveaux and Olivier Tournilhac and Michelle Imbert and Henry Rochant and Michel Goossens},
  journal={Human mutation},
  year={1994},
  volume={3 2},
  pages={126-32}
}
We report the set-up of a denaturant gradient gel electrophoresis (DGGE) assay to screen for mutations in the whole coding sequence of the p53 gene. These DGGE experimental conditions were applied to the analysis of the p53 gene in acute leukemias. Forty adults with acute myelogenous leukemia (AML) and 21 with acute lymphoid leukemia (ALL) were investigated. Eleven of the AML patients were investigated at the time of the initial diagnosis and at relapse. In contrast with most reports based on… CONTINUE READING