Excluded volume as a determinant of macromolecular structure and reactivity

@article{Minton1981ExcludedVA,
  title={Excluded volume as a determinant of macromolecular structure and reactivity},
  author={Allen P Minton},
  journal={Biopolymers},
  year={1981},
  volume={20}
}
  • A. Minton
  • Published 1 October 1981
  • Chemistry
  • Biopolymers
The effect of excluded volume on the thermodynamic activity of globular macromolecules and macromolecular complexes in solution is studied in the hard‐particle approximation. Activity coefficients are calculated as a function of the fraction of total volume occupied by macromolecules using relations obtained from scaled particle and lattice models. Significant and readily observable effects are predicted to occur as the fraction of volume occupied by globular macromolecules increases, including… 
The influence of macromolecular crowding on thermodynamic activity: Solubility and dimerization constants for spherical and dumbbell‐shaped molecules in a hard‐sphere mixture
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Experimental studies of the effect of ‘inert’ macromolecules on protein structure and/or function are reviewed, and it is found that under favorable circumstances the simplified models can provide a satisfactory semiquantitative description of the data.
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Sedimentation equilibria in solutions containing either a nonassociating solute or a solute that self‐associates according to several different schemes are simulated using the derived relations and the results are presented in terms of the dependence of apparent weight‐average molecular weight upon solute concentration.
Analysis of the size dependence of macromolecular crowding shows that smaller is better
  • K. Sharp
  • Chemistry, Medicine
    Proceedings of the National Academy of Sciences
  • 2015
TLDR
Crowding, a nonspecific phenomenon believed to arise from the large excluded volume of these macromolecules, has been studied extensively by experimental and theoretical methods but the relevant theory has not been applied consistently.
Theoretical investigation of the kinetics and thermodynamics of conformational equilibrium in crowded medium
Macromolecular crowding alters the thermodynamic activities and kinetics of biochemical processes through an excluded volume effect. A theoretical framework, comprised of the Monte Carlo, extended
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