Excited-state intermolecular proton transfer of firefly luciferin IV. Temperature and pH dependence.

@article{Erez2011ExcitedstateIP,
  title={Excited-state intermolecular proton transfer of firefly luciferin IV. Temperature and pH dependence.},
  author={Yuval Erez and Itay Presiado and Rinat Gepshtein and Dan Huppert},
  journal={The journal of physical chemistry. A},
  year={2011},
  volume={115 9},
  pages={
          1617-26
        }
}
Time-resolved emission as well as steady-state UV-vis techniques were employed to study the photoprotolytic processes that d-luciferin, the natural substrate of the firefly luciferase, undergoes in both acidic aqueous solutions and ice. The emission spectrum of D-luciferin in a 20 mM HCl aqueous solution or higher has an additional emission band at 590 nm red-shifted with respect to the strongest emission band positioned at 530 nm of the deprotonated NRO(-*) form in a pH-neutral aqueous… CONTINUE READING