Excision of foreign gene product with cathepsin D in chicken hepatoma cell line.

  title={Excision of foreign gene product with cathepsin D in chicken hepatoma cell line.},
  author={Masaharu Sato and Tsuyoshi Kawashima and Masayoshi Aosasa and Hiroyuki Horiuchi and Shuichi Furusawa and Haruo Matsuda},
  journal={Biochemical and biophysical research communications},
  volume={330 2},
To easily and rapidly recover exogenous gene products from chicken egg yolk, we constructed pVTG-catD (VTG, vitellogenin; catD, cathepsin D), a vector cassette carrying two catD-recognition signal peptides (catD-RSPs) in addition to the cloning site. An enhanced green fluorescence protein (EGFP)-encoding DNA fragment was ligated into the pVTG-catD. When the resultant construct pVTG-EGFP-catD containing histidine- and myc-tags was transfected into the chicken hepatoma cell line LMH, EGFP… 
Establishment and Characterization of a Novel Tissue-specific DNA Construct and Culture System with Potential for Avian Bioreactor Generation
This work establishes and characterize a DNA construct capable of showing the ability of the promoter to drive expression of a reporting gene in a tissue-specific manner and in a way that closely resembles physiologic regulation of vitellogenin, making it an ideal candidate to be used in the future for generation of avian bioreactors.
Comparative proteomic analysis of chicken, duck, and quail egg yolks
Comparative 2D gel electrophoresis patterns of chicken, duck, and quail revealed that some protein-specific regions on gels could be used for authentic identification of poultry eggs and showed some new sights in egg yolk nutrients related to certain properties and functions.


Development of transgenic chickens expressing enhanced green fluorescent protein.
  • M. Kwon, B. Koo, +7 authors Teoan Kim
  • Biology, Medicine
    Biochemical and biophysical research communications
  • 2004
The results show the exceptional versatile effectiveness of the EGFP gene as a marker in the gene expression-related studies which therefore would be very helpful in establishing a useful transgenic chicken model system for studies on embryo development and for efficient production of transgenic chickens as bioreactors.
Expression of exogenous protein in the egg white of transgenic chickens
The inserted transgene encoding a secreted protein, β-lactamase, under the control of the ubiquitous cytomegalovirus promoter supported the potential of the hen as a bioreactor for the production of commercially valuable, biologically active proteins in egg white.
Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells
This work mutated, by gene targeting, the endogenous hypoxanthine phosphoribosyl transferase (HPRT) gene in mouse embryo-derived stem (ES) cells and compared the gene-targeting efficiencies of two classes of neor-Hprt recombinant vectors.
Development of transgenic chickens expressing bacterial β‐galactosidase
The replication‐defective retroviral SNTZ vector carrying the lacZ gene with a nuclear localized signal was injected into the subgerminal cavity of freshly laid eggs, and the chickens were screened for the LacZ gene by using the polymerase chain reaction.
Solubilization and characterization of the chicken oocyte vitellogenin receptor.
Antibodies directed against the mammalian receptor for low-density lipop protein showed cross-reactivity with the chicken oocyte VTG receptor, raising the possibility that lipoprotein receptors in birds are structurally related to those in mammalian species.
Chicken Leukemia Inhibitory Factor Maintains Chicken Embryonic Stem Cells in the Undifferentiated State*
It is concluded that chicken LIF is able to maintain chicken ES cell cultures in the undifferentiated state and encodes a protein with ∼40% sequence identity to mouse LIF.
Transgenic animal bioreactors
The generation of transgenic farm animals has become recently easier mainly with the technique of animal cloning using transfected somatic cells as nuclear donor, but this point remains a limitation as far as cost is concerned.
Efficient production of germline transgenic chickens using lentiviral vectors
It is demonstrated that lentiviral vectors can be used to generate transgenic lines with an efficiency in the order of 100‐fold higher than any previously published method, with no detectable silencing of transgene expression between generations.
Genetic transformation of mouse embryos by microinjection of purified DNA.
The results demonstrate that genes can be introduced into the mouse genome by direct insertion into the nuclei of early embryos by microinjected into pronuclei of fertilized mouse oocytes.
Biologically Active Human Interferon α-2b Produced in the Egg White of Transgenic Hens
The expression of a glycosylated human protein, interferon α-2b (hIFN), in the egg white of transgenic hens shows the potential of the hen as a bioreactor for the production of commercially valuable, biologically active, and glycoslyated proteins in egg white.