Excision and transfer of the Mesorhizobium loti R7A symbiosis island requires an integrase IntS, a novel recombination directionality factor RdfS, and a putative relaxase RlxS

@article{Ramsay2006ExcisionAT,
  title={Excision and transfer of the Mesorhizobium loti R7A symbiosis island requires an integrase IntS, a novel recombination directionality factor RdfS, and a putative relaxase RlxS},
  author={J. Ramsay and J. Sullivan and Gabriella S. Stuart and I. Lamont and C. Ronson},
  journal={Molecular Microbiology},
  year={2006},
  volume={62}
}
The Mesorhizobium loti strain R7A symbiosis island is an Integrative Conjugative Element (ICE), herein termed ICEMlSymR7A, which integrates into a phetRNA gene. Integration reconstructs the phetRNA gene at one junction with the core chromosome, and a direct repeat of the 3‐prime 17 bp of the gene is formed at the other junction. We show that the ICEMlSymR7AintS gene, which encodes an integrase of the phage P4 family, is required for integration and excision of the island. Excision also depended… Expand
A LuxRI‐family regulatory system controls excision and transfer of the Mesorhizobium loti strain R7A symbiosis island by activating expression of two conserved hypothetical genes
TLDR
The data suggest that derepressed TraR in conjunction with TraI1‐synthesized 3‐oxo‐C6‐HSL regulates excision and transfer of ICEMlSymR7A through expression of msi172 and msi171. Expand
Delineation of the integrase-attachment and origin-of-transfer regions of the symbiosis island ICEMlSymR7A.
TLDR
This work defines two critical cis-acting regions required for excision and transfer of ICEMlSymR7A and related ICEs. Expand
A widely conserved molecular switch controls quorum sensing and symbiosis island transfer in Mesorhizobium loti through expression of a novel antiactivator
TLDR
QseM, a widely conserved ICE‐encoded protein, is an antiactivator of TraR, suggesting that QseC and QseM comprise a bimodal switch that restricts quorum sensing and ICEMlSymR7A transfer to a small proportion of cells in the population. Expand
Genomic Islands in Sinorhizobium meliloti Rm1021, Nitrogen-Fixing Symbiont of Alfalfa
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In silico analysis of open reading frames of genomic islands of Sinorhizobium meliloti Rm1021 was performed, it was demonstrated that genomic islands had mosaic structure, in which blocks of functional genes, IS-elements and noncoding RNA alternated. Expand
Genome sequence of the Lotus corniculatus microsymbiont Mesorhizobium loti strain R88B
TLDR
The genome of M. loti strain R88B contains a single scaffold of size 7,195,110 bp which encodes 6,950 protein-coding genes and 66 RNA-only encoding genes and three acquired loci that together allow the utilization of the siderophore ferrichrome. Expand
Characterisation of the symbiosis ICE and accessory plasmid of Mesorhizobium ciceri
TLDR
The aims of this thesis were to determine the symbiotic role of the accessory plasmid, pMc1192 in M. ciceri CC1192 and to investigate means of producing an ICE-devoid M. arietinum strain, which may have a role in nitrogen fixation. Expand
Genome sequence of the Lotus spp. microsymbiont Mesorhizobium loti strain R7A
TLDR
The genome of M. loti strain R7A does not harbor any plasmids and contains a single scaffold of size 6,529,530 bp which encodes 6,323 protein-coding genes and 75 RNA-only encoding genes. Expand
The NifA-RpoN Regulon of Mesorhizobium loti Strain R7A and Its Symbiotic Activation by a Novel LacI/GalR-Family Regulator
TLDR
Many genes encoded on ICEMlSymR7A were strongly expressed in nodules but not free-living rhizobia, but were not essential for symbiotic nitrogen fixation. Expand
Identification and characterization of int (integrase), xis (excisionase) and chromosomal attachment sites of the integrative and conjugative element ICEBs1 of Bacillus subtilis
TLDR
It is found that in the absence of the primary bacterial attachment site (attB) in trnS‐leu2, ICEBs1 integrated in secondary attachment sites that are similar to a 17 bp sequence in attB. Expand
ICEAfe1, an Actively Excising Genetic Element from the Biomining Bacterium Acidithiobacillus ferrooxidans
TLDR
Transfer of ICEAfe1 may provide selective advantages to other acidophiles in this ecological niche through dissemination of gene clusters expressing transfer RNAs, CRISPRs, and exopolysaccharide biosynthesis enzymes, probably by modification of translation efficiency, resistance to bacteriophage infection and biofilm formation, respectively. Expand
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References

SHOWING 1-10 OF 59 REFERENCES
Comparative Sequence Analysis of the Symbiosis Island of Mesorhizobium loti Strain R7A
TLDR
The nucleotide sequence of the R7A island is determined and its deduced genetic complement is compared with that reported for the 611-kb putative symbiosis island of M. loti strain MAFF303099, indicating that the island lacks plasmid replication genes, suggesting that it is a site-specific conjugative transposon. Expand
Excision of the high‐pathogenicity island of Yersinia pseudotuberculosis requires the combined actions of its cognate integrase and Hef, a new recombination directionality factor
TLDR
It is shown that, upon excision, the Yersinia high‐pathogenicity island (HPI) forms an episomal circular molecule and has the ability to excise from the chromosome, circularize and reintegrate itself, either in the same location or in another asn tRNA copy. Expand
The bio operon on the acquired symbiosis island of Mesorhizobium sp. strain R7A includes a novel gene involved in pimeloyl-CoA synthesis.
TLDR
In this work, a 13.2 kb region of the island that converts the nonsymbionts to nicotinate and biotin prototrophy was characterized and the rationale for the vitamin biosynthetic loci being located on an acquired genetic element that is absent from nonsYmbiotic mesorhizobia remains to be determined. Expand
Evolution of rhizobia by acquisition of a 500-kb symbiosis island that integrates into a phe-tRNA gene.
  • J. Sullivan, C. Ronson
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1998
TLDR
The chromosomal symbiotic element of M. loti strain ICMP3153 is transmissible in laboratory matings to at least three genomic species of nonsymbiotic mesorhizobia and is termed a symbiosis island on the basis of its many similarities to pathogenicity islands. Expand
Symbiotic phenotypes and translocated effector proteins of the Mesorhizobium loti strain R7A VirB/D4 type IV secretion system
TLDR
The heterologous translocation of rhizobial proteins by the Agrobacterium VirB/D4 T4SS is the first demonstration that rhzobial effector proteins are translocated into plant cells and confirms functional conservation between the M. loti and A. tumefaciens T4 SS strains. Expand
Regulated site‐specific recombination of the she pathogenicity island of Shigella flexneri
TLDR
It is demonstrated that spontaneous, precise excision of the element from its integration site in the 3′ terminus of the pheV tRNA gene is mediated by an integrase gene (int) and a gene designated rox (regulator of excision), both of which are carried on the she PAI. Expand
Role of pathogenicity island‐associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536
TLDR
Although all mediated by P4‐like integrases, the activity of the PAI excision machinery is most often restricted to its cognate island, and this work demonstrates for the first time the existence of a cross‐talk betweenIntegrases of different PAIs and shows that this cross-talk is unidirectional. Expand
Int-B13, an Unusual Site-Specific Recombinase of the Bacteriophage P4 Integrase Family, Is Responsible for Chromosomal Insertion of the 105-Kilobase clc Element ofPseudomonas sp. Strain B13
TLDR
The localization and nucleotide sequence of the integrase gene and the activity of theIntegrase gene product in mediating site-specific integration are reported here and demonstrated sequence specificity of the integration. Expand
The Biphenyl- and 4-Chlorobiphenyl-Catabolic Transposon Tn4371, a Member of a New Family of Genomic Islands Related to IncP and Ti Plasmids
TLDR
Tn4371 appears to represent a new family of potentially mobile genomic islands with a broad host range since they reside in a wide range of soil proteobacteria, including plant pathogens. Expand
The clc Element of Pseudomonas sp. Strain B13, a Genomic Island with Various Catabolic Properties
TLDR
The complete nucleotide sequence of the clc element is reported, demonstrating the unique catabolic properties while showing its relatedness to genomic islands and integrative and conjugative elements rather than to other known catabolic plasmids. Expand
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