Excision and integration of a self-transmissible replicon of Streptomyces ambofaciens.

@article{Simonet1987ExcisionAI,
  title={Excision and integration of a self-transmissible replicon of Streptomyces ambofaciens.},
  author={Julien Simonet and Fr{\'e}d{\'e}ric Boccard and Jean Luc Pernodet and Josette Gagnat and Michel Gu{\'e}rineau},
  journal={Gene},
  year={1987},
  volume={59 1},
  pages={137-44}
}
When Streptomyces ambofaciens OSF was crossed with the plasmid-free Streptomyces lividans TK24, almost all S. lividans exconjugants contained the free 11.1-kb plasmid pOS1. Southern hybridizations showed that pOS1 was derived from the integrated copy of previously recognized plasmid pSAM2 present in strain OSF. A shorter derivative of pOS1 was constructed carrying the tsr gene in a non-essential region, and this pOS7 plasmid was used in transformation experiments with protoplasts of S… CONTINUE READING