• Chemistry, Medicine
• Published in Biochemistry 2000
• DOI:10.1021/bi991423k

Exchanging cofactors in the core antennae from purple bacteria: structure and properties of Zn-bacteriopheophytin-containing LH1.

@article{Lapouge2000ExchangingCI,
  title={Exchanging cofactors in the core antennae from purple bacteria: structure and properties of Zn-bacteriopheophytin-containing LH1.},
  author={Karine Lapouge and Arne N{\"a}veke and Bruno Robert and Hugo Scheer and James N. Sturgis},
  journal={Biochemistry},
  year={2000},
  volume={39 5},
  pages={
          1091-9
        }
}

• Karine Lapouge, Arne Näveke, +2 authors James N. Sturgis

The core light-harvesting LH1 complex of Rhodospirillum rubrum consists of an assembly of membrane-spanning alpha and beta polypeptides, each of which binds one bacteriochlorophyll (BChl) a molecule. In this work, we describe a technique that allows the replacement of the natural, Mg BChl a cofactors present in this protein by Zn-bacteriopheophytin (Zn-Bpheo). This technique makes use of the well-characterized, reversible dissociation of LH1 induced by the detergent beta-octylglucoside… CONTINUE READING

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