Exchange Activity Is Localized in the T-Tubules of Rat Ventricular Myocytes

  title={Exchange Activity Is Localized in the T-Tubules of Rat Ventricular Myocytes},
  author={Zhaoxiang Yang and Caroline Pascarel and Derek Steele and Kimiaki Komukai and Fabien Brette and Clive H Orchard},
Detubulation of rat ventricular myocytes has been used to investigate the role of the t-tubules in Ca cycling during excitation-contraction coupling in rat ventricular myocytes. Ca was monitored using fluo-3 and confocal microscopy. In control myocytes, electrical stimulation caused a spatially uniform increase in intracellular [Ca ] across the cell width. After detubulation, [Ca ] rose initially at the cell periphery and then propagated into the center of the cell. Application of caffeine to… CONTINUE READING


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