Excessive sarcoplasmic/endoplasmic reticulum Ca2+-ATPase expression causes increased sarcoplasmic reticulum Ca2+ uptake but decreases myocyte shortening.

@article{Teucher2004ExcessiveSR,
  title={Excessive sarcoplasmic/endoplasmic reticulum Ca2+-ATPase expression causes increased sarcoplasmic reticulum Ca2+ uptake but decreases myocyte shortening.},
  author={Nils Teucher and Juergen Prestle and Tim Seidler and Susan L. Currie and Elspeth E B Elliott and Deborah F Reynolds and Peter Schott and Stefan Wagner and Harald Kogler and Giuseppe Inesi and Donald M. Bers and Gerd Hasenfuss and Godfrey L. Smith},
  journal={Circulation},
  year={2004},
  volume={110 23},
  pages={3553-9}
}
BACKGROUND Increasing sarcoplasmic/endoplasmic reticulum (SR) Ca2+-ATPase (SERCA) uptake activity is a promising therapeutic approach for heart failure. We investigated the effects of different levels of SERCA1a expression on contractility and Ca2+ cycling. We tested whether increased SERCA1a expression levels enhance myocyte contractility in a gene-dose-dependent manner. METHODS AND RESULTS Rabbit isolated cardiomyocytes were transfected at different multiplicities of infection (MOIs) with… CONTINUE READING