Excessive function of peripheral blood neutrophils from patients with Behçet's disease and from HLA-B51 transgenic mice.

Abstract

OBJECTIVE To elucidate the role played by HLA-B51 in the neutrophil hyperfunction of Behçet's disease, we determined the superoxide production by purified peripheral blood neutrophils from Behçet's disease patients, from HLA-B51 positive healthy individuals, and from HLA-B51 transgenic mice. METHODS Neutrophil function was evaluated by flow cytometric analysis, detecting the conversion of 2',7'-dichlorofluorescin diacetate into dichloroflurescein, induced by superoxide in the neutrophils. RESULTS A significant correlation between the neutrophil hyperfunction and the possession of HLA-B51 phenotype, regardless of the presence of the disease, was observed in humans. FMLP-stimulated neutrophils (without in vitro priming) from HLA-B51 transgenic mice, but not those from HLA-B35 transgenic mice or from nontransgenic mice, produced substantial amounts of superoxide. CONCLUSION The HLA-B51 molecule itself may be responsible, at least in part, for neutrophil hyperfunction in Behçet's disease.

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@article{Takeno1995ExcessiveFO, title={Excessive function of peripheral blood neutrophils from patients with Behçet's disease and from HLA-B51 transgenic mice.}, author={Mhoso Takeno and A I Kariyone and Naohide Yamashita and Masafumi Takiguchi and Yutaka Mizushima and Hidetoshi Kaneoka and Tsuyoshi Sakane}, journal={Arthritis and rheumatism}, year={1995}, volume={38 3}, pages={426-33} }