Examination of Potential Inhibitors of Hepatitis A Virus Uncoating

@article{Bishop1998ExaminationOP,
  title={Examination of Potential Inhibitors of Hepatitis A Virus Uncoating},
  author={Naomi E. Bishop},
  journal={Intervirology},
  year={1998},
  volume={41},
  pages={261 - 271}
}
  • N. Bishop
  • Published 1998
  • Medicine, Biology
  • Intervirology
Hepatitis A virus (HAV) replication in BS-C-1 cells was studied in the presence of ten potential uncoating inhibitors. Strong inhibition of HAV replication was only observed in the presence of the phenothiazine compound chlorpromazine and the lysosomotropic agent chloroquine, but not by other lysosomotropic agents. Chlorpromazine and chloroquine were found to prevent virus uncoating. Chlorpromazine is known to inhibit endocytosis of non- clathrin-coated vesicles. Chloroquine is a weak base… Expand
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References

SHOWING 1-10 OF 66 REFERENCES
Inhibition of uncoating of poliovirus by arildone, a new antiviral drug.
TLDR
Arildone inhibits uncoating of poliovirus and thereby prevents virus-induced shutoff of host cell protein synthesis, and possible mechanisms by which arildone interacts with the poliov virus icosahedral capsid to prevent uncoatering are discussed. Expand
Early steps in FMDV replication: further analysis on the effects of chloroquine.
TLDR
It is demonstrated that a decrease in the environmental pH counteracts the effect of chloroquine indicating that viral disruption is a low-pH cell-mediated process and that prelysosomal vesicles represent the putative site for uncoating. Expand
Early interactions of hepatitis A virus with cultured cells: viral elution and the effect of pH and calcium ions
TLDR
The results suggests that the major effect of calcium in promoting HAV-receptor interactions is through a direct effect on the conformation of the viral capsid. Expand
Hepatitis A virus attachment to cultured cell lines.
TLDR
It is demonstrated that HAV has a calcium-dependent receptor on cultured cell lines and suggested that the HAV binding region does not involve an RGD sequence or the Hav immunodominant neutralization site. Expand
Mechanism of entry into the cytosol of poliovirus type 1: requirement for low pH
TLDR
The results indicate that acidification somehow facilitates the entry of the virus RNA into the cytosol and that under normal conditions the entry occurs from intracellular acidic vesicles. Expand
Kinetics of poliovirus uncoating in HeLa cells in a nonacidic environment
TLDR
A mechanism of poliovirus uncoating which is independent of low pH is proposed, which could be attributed to a delay of virus entry into cells after treatment with methylamine and monensin, whereas chloroquine stabilized the viral capsid itself. Expand
Influence of twenty potentially antiviral substances on in vitro multiplication of hepatitis A virus.
TLDR
Although no promising candidates for antiviral treatment of hepatitis A have emerged from the present study, the assay model described here would seem useful in the screening of substances with inhibitory effects on HAV. Expand
The site of attachment in human rhinovirus 14 for antiviral agents that inhibit uncoating.
TLDR
Two compounds are structurally related, antiviral compounds that inhibit the replication of rhino (common cold) viruses and related picornaviruses and prevent the pH-mediated uncoating of the viral RNA. Expand
Parameters influencing the attachment of hepatitis A virus to a variety of continuous cell lines.
TLDR
The data show that the components of serum which inhibit attachment are of high Mr and that the serum glycoprotein, alpha 2-macroglobulin, can partly mimic the inhibitory effect of whole serum. Expand
Suramin inhibits in vitro infection by duck hepatitis B virus, Rous sarcoma virus, and hepatitis delta virus.
TLDR
It is found that suramin blocked infection by hepatitis delta virus, an RNA virus that is not known to employ reverse transcriptase during the initiation of infection, and this results are consistent with the hypothesis thatSuramin acted by blocking virus uptake or uncoating. Expand
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