Ex vivo transfection of trout pronephros leukocytes, a model for cell culture screening of fish DNA vaccine candidates.

@article{OrtegaVillaizan2012ExVT,
  title={Ex vivo transfection of trout pronephros leukocytes, a model for cell culture screening of fish DNA vaccine candidates.},
  author={Mar{\'i}a Del Mar Ortega-Villaizan and Alicia Mart{\'i}nez-L{\'o}pez and Pablo Garc{\'i}a-Valtanen and Ver{\'o}nica Chico and Luis Perez and Julio Morales Coll and Amparo Estepa},
  journal={Vaccine},
  year={2012},
  volume={30 41},
  pages={5983-90}
}
DNA vaccination opened a new era in controlling and preventing viral diseases since DNA vaccines have shown to be very efficacious where some conventional vaccines have failed, as it occurs in the case of the vaccines against fish novirhabdoviruses. However, there is a big lack of in vitro model assays with immune-related cells for preliminary screening of in vivo DNA vaccine candidates. In an attempt to solve this problem, rainbow trout pronephros cells in early primary culture were… CONTINUE READING