Ex vivo stimulation and expansion of both CD4(+) and CD8(+) T cells from peripheral blood mononuclear cells of human cytomegalovirus-seropositive blood donors by using a soluble recombinant chimeric protein, IE1-pp65.

@article{VazSantiago2001ExVS,
  title={Ex vivo stimulation and expansion of both CD4(+) and CD8(+) T cells from peripheral blood mononuclear cells of human cytomegalovirus-seropositive blood donors by using a soluble recombinant chimeric protein, IE1-pp65.},
  author={J Vaz-Santiago and Jacqueline Lul{\'e} and Pierre S Rohrlich and Caroline Jacquier and N. S. Gibert and Emmanuelle Le Roy and Didier Betbeder and J L Davignon and Christian Davrinche},
  journal={Journal of virology},
  year={2001},
  volume={75 17},
  pages={7840-7}
}
The transfer of anti-human cytomegalovirus (HCMV) effector T cells to allogeneic bone marrow recipients results in protection from HCMV disease associated with transplantation, suggesting the direct control of CMV replication by T cells. IE1 and pp65 proteins, both targets of CD4(+) and CD8(+) T cells, are considered the best candidates for immunotherapy and vaccine design against HCMV. In this report, we describe the purification of a 165-kDa chimeric protein, IE1-pp65, and its use for in… CONTINUE READING

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