Ex vivo red blood cell hemolysis assay for the evaluation of pH-responsive endosomolytic agents for cytosolic delivery of biomacromolecular drugs.

@article{Evans2013ExVR,
  title={Ex vivo red blood cell hemolysis assay for the evaluation of pH-responsive endosomolytic agents for cytosolic delivery of biomacromolecular drugs.},
  author={Brian C. Evans and Christopher E. Nelson and Shann C. S. Yu and Kelsey Ross Beavers and Arnold J Kim and Hongmei Li and Heather McKay Nelson and Todd D Giorgio and Craig L Duvall},
  journal={Journal of visualized experiments : JoVE},
  year={2013},
  volume={73},
  pages={
          e50166
        }
}
Phospholipid bilayers that constitute endo-lysosomal vesicles can pose a barrier to delivery of biologic drugs to intracellular targets. To overcome this barrier, a number of synthetic drug carriers have been engineered to actively disrupt the endosomal membrane and deliver cargo into the cytoplasm. Here, we describe the hemolysis assay, which can be used as rapid, high-throughput screen for the cytocompatibility and endosomolytic activity of intracellular drug delivery systems. In the… CONTINUE READING

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