Ex vivo expansion and gene therapy using cord blood CD34+ cells.

@article{Moore1993ExVE,
  title={Ex vivo expansion and gene therapy using cord blood CD34+ cells.},
  author={Malcolm A. S. Moore},
  journal={Journal of hematotherapy},
  year={1993},
  volume={2 2},
  pages={221-4}
}
CD34+ cells can be efficiently isolated from cord blood by sequential Percoll, Ficoll-Hypaque, and immunomagnetic bead separation. This population, containing up to 25% progenitor cells and 0.2-1% stem cells, defined by a long-term culture-initiating cell (LTC-initiating cell) assay, can be expanded in vitro in the presence of a specific combination of cytokines resulting in a > 100,000-fold increase in cells, a > 2,000-fold increase in progenitors, and an 18- to 20-fold increase in LTC… CONTINUE READING