Ex vivo IFN-gamma secretion by circulating CD8 T lymphocytes: implications of a novel approach for T cell monitoring in infectious and malignant diseases.

@article{Pittet2001ExVI,
  title={Ex vivo IFN-gamma secretion by circulating CD8 T lymphocytes: implications of a novel approach for T cell monitoring in infectious and malignant diseases.},
  author={Mik{\"a}el J. Pittet and Alfred Zippelius and Daniel E Speiser and Mario Assenmacher and Philippe Guillaume and Danila Valmori and Danielle Li{\'e}nard and Ferdy Lejeune and Jean Charles Cerottini and Pedro Romero},
  journal={Journal of immunology},
  year={2001},
  volume={166 12},
  pages={7634-40}
}
To elucidate the functional heterogeneity of Ag-specific T lymphocyte populations, we combined labeling of lymphocytes with MHC/peptide tetramers and a cell surface affinity matrix for IFN-gamma. Magnetic cell sorting of IFN-gamma-positive lymphocytes allowed the selective enrichment and identification of live Ag-specific cytokine-secreting cells by flow cytometry. Naive, memory, and effector Ag-specific populations were evaluated in healthy HLA-A2 individuals. Significant fractions of… CONTINUE READING
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