Ex vivo CF diagnosis by intestinal current measurements (ICM) in small aperture, circulating Ussing chambers.

@article{Jonge2004ExVC,
  title={Ex vivo CF diagnosis by intestinal current measurements (ICM) in small aperture, circulating Ussing chambers.},
  author={Hugo R. de Jonge and Manfred Ballmann and Henk J. Veeze and Inez Bronsveld and Frauke Stanke and Burkhard T{\"u}mmler and Maarten Sinaasappel},
  journal={Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society},
  year={2004},
  volume={3 Suppl 2},
  pages={159-63}
}
Intestinal current measurements (ICM) on rectal suction biopsies are a tool for the ex vivo diagnosis of classical and atypical cystic fibrosis (CF). We present the basic ICM protocol, typical tracings and their interpretation. The ICM technique allows the registration of CF-induced changes in electrogenic transepithelial ion transport (Cl-, HCO3-, K+) in a Cl- secretory epithelium, and on the basis of pharmacological criteria, is able to discriminate between CFTR-mediated Cl- secretion and… CONTINUE READING