Ex situ conservation of Phyllanthus fraternus Webster and evaluation of genetic fidelity in regenerates using DNA-based molecular marker.

@article{Upadhyay2014ExSC,
  title={Ex situ conservation of Phyllanthus fraternus Webster and evaluation of genetic fidelity in regenerates using DNA-based molecular marker.},
  author={Richa Upadhyay and Sarvesh Pratap Kashyap and Chandra Shekhar Singh and Kavindra Nath Tiwari and Karuna B. Singh and Major Jai Inder Singh},
  journal={Applied biochemistry and biotechnology},
  year={2014},
  volume={174 6},
  pages={2195-208}
}
Germplasm storage of Phyllanthus fraternus by using synseed technology has been optimized. Synseeds were prepared from nodal segments taken from in vitro-grown plantlets. An encapsulation matrix of 3 % sodium alginate and 100 mM calcium chloride with polymerization duration up to 15 min was found most suitable for synseed formation. Maximum plantlet conversion (92.5 ± 2.5 %) was obtained on a growth regulator-free ½-strength solid Murashige and Skoog (MS) medium. Multiple shoot proliferation… CONTINUE READING