Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast

@inproceedings{GarcaRuiz2010EvolvingTI,
  title={Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast},
  author={Eva Garc{\'i}a-Ruiz and Diana M. Mate and A. Ortega Ballesteros and {\'A}ngel T Martinez and Miguel Alcalde},
  booktitle={Microbial cell factories},
  year={2010}
}
BACKGROUND In the picture of a laboratory evolution experiment, to improve the thermostability whilst maintaining the activity requires of suitable procedures to generate diversity in combination with robust high-throughput protocols. The current work describes how to achieve this goal by engineering ligninolytic oxidoreductases (a high-redox potential laccase -HRPL- and a versatile peroxidase, -VP-) functionally expressed in Saccharomyces cerevisiae. RESULTS Taking advantage of the… CONTINUE READING
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