Evolutionary relationship between different subgroups of restriction endonucleases.

@article{Pingoud2002EvolutionaryRB,
  title={Evolutionary relationship between different subgroups of restriction endonucleases.},
  author={Vera Pingoud and Elena Kubareva and Gudrun Stengel and Peter Friedhoff and Janusz M. Bujnicki and Claus Urbanke and A. E. Sudina and Alfred Pingoud},
  journal={The Journal of biological chemistry},
  year={2002},
  volume={277 16},
  pages={14306-14}
}
The type II restriction endonuclease SsoII shows sequence similarity with 10 other restriction endonucleases, among them the type IIE restriction endonuclease EcoRII, which requires binding to an effector site for efficient DNA cleavage, and the type IIF restriction endonuclease NgoMIV, which is active as a homotetramer and cleaves DNA with two recognition sites in a concerted reaction. We show here that SsoII is an orthodox type II enzyme, which is active as a homodimer and does not require… CONTINUE READING