Evolutionary optimization of fluorescent proteins for intracellular FRET

@article{Nguyen2005EvolutionaryOO,
  title={Evolutionary optimization of fluorescent proteins for intracellular FRET},
  author={Annalee W. Nguyen and Patrick S. Daugherty},
  journal={Nature Biotechnology},
  year={2005},
  volume={23},
  pages={355-360}
}
Fluorescent proteins that exhibit Förster resonance energy transfer (FRET) have made a strong impact as they enable measurement of molecular-scale distances through changes in fluorescence. FRET-based approaches have enabled otherwise intractable measurements of molecular concentrations, binding interactions and catalytic activity, but are limited by the dynamic range and sensitivity of the donor-acceptor pair. To address this problem, we applied a quantitative evolutionary strategy using… CONTINUE READING
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