LARP1 is a major phosphorylation substrate of mTORC1
- Biology, ChemistrybioRxiv
This study examined the phosphorylation of a recently identified target of mTORC1: La-related protein 1 (LARP1), a member of the LARP superfamily, and revealed LARP1 as a major substrate of m TORC1.
Phenotypic plasticity in the symbiotic cnidarian Anemonia viridis : stress response at multiple levels of structural complexity
This work investigated the phenotypic plasticity of the non-calcifying symbiotic cnidarian Anemonia viridis at multiple levels of structural complexity, in vivo and in vitro, and validated the use of this new tool to the in vitro study of stress response at the cellular level.
Looking through the Lens of the Ribosome Biogenesis Evolutionary History: Possible Implications for Archaeal Phylogeny and Eukaryogenesis
- BiologyMolecular biology and evolution
It is proposed to exploit additional functional hallmarks of ribosome biogenesis to help disentangle competing evolutionary hypotheses and illustrate and discuss how function-aware phylogenetic framework can contribute to refining the understanding of archaeal phylogeny and the origin of eukaryotic cells.
Net Charges of the Ribosomal Proteins of the S10 and spc Clusters of Halophiles Are Inversely Related to the Degree of Halotolerance
The net charges of ribosomal proteins (r-proteins) that comprise the S10-spc cluster show an inverse relationship with the halophilicity/halotolerance levels in both bacteria and archaea, extended to non-archaeal halophilic bacteria, eukaryotes, and halotolerant archaea.
The Peptidyl Transferase Center: a Window to the Past
- BiologyMicrobiology and molecular biology reviews : MMBR
A detailed review of the literature focused on the nature of the extant PTC and its proposed ancestor, the proto-ribosome is presented.
A Genomic Catalog of Stress Response Genes in Anaerobic Fungi for Applications in Bioproduction
- Biology, EngineeringFrontiers in Fungal Biology
This work delineate the unique stress response of anaerobic fungi, which is an important step toward their development as a biotechnology platform to produce enzymes and valuable biomolecules.
A conserved choreography of mRNAs at centrosomes reveals a localization mechanism involving active polysome transport
High-throughput smFISH is used to screen centrosomal protein-coding genes, and 8 human mRNAs accumulating at centrosomes are described, indicating a finely regulated translational program at Centrosomes.
Orthopoxvirus K3 orthologs show virus- and host-specific inhibition of the antiviral protein kinase PKR
It is shown that even closely related inhibitors of an antiviral protein can vary dramatically in their inhibitory potential, and cautions that results from host-virus interaction studies of a prototypic virus genus member cannot necessarily be extrapolated to other viruses in the same genus without experimental verification.
"The structure of human GCN2 reveals a parallel, back-to-back kinase dimer with a plastic DFG activation loop motif".
- Biology, ChemistryThe Biochemical journal
The first crystal structures of human GCN2 kinase domain in complex with two inhibitors of different size, shape, and chemical scaffold are reported, consistent with the hypothesis that theGCN2 Kinase Domain forms an antiparallel inactive dimer until uncharged t-RNA binds to it and triggers conformational changes that shifts the equilibrium to the active parallel dimer.
A novel eIF4E interacting protein that forms non-canonical translation initiation complexes
- BiologyNature Plants
Characterizing an eIF4E-interacting protein, CERES, as a non-canonical translation initiation factor that modulates translation in plants is characterized.
SHOWING 1-10 OF 1,813 REFERENCES
p70 S6K1 nuclear localization depends on its mTOR-mediated phosphorylation at T389, but not on its kinase activity towards S6
- Biology, ChemistryAmino Acids
It is demonstrated that the proper nucleocytoplasmic localization of this kinase depends on its mTOR-mediated phosphorylation but not on its kinase activity, which provides important insights into the regulation of p70 S6K1 and allow a more detailed understanding of subcellular enzyme localization processes.
Why do proteins use selenocysteine instead of cysteine?
- Chemistry, BiologyAmino Acids
The kinetic and thermodynamic properties of cysteine with its selenium-containing analogon, selenocysteine, are compared to find out whether it has a protective effect in proteins or can be used to tune electrode, or redox, potentials.
A series of eIF4E alleles at the Bc-3 locus are associated with recessive resistance to Clover yellow vein virus in common bean
- Theoretical and Applied Genetics
KASPar allele-specific SNP genotyping assays were developed and demonstrated their ability to accurately detect and differentiate all of the PveIF4E haplotypes present in the germplasm, allelism testing, and in three separate segregating populations and contribute to an enhanced understanding and accessibility of the important potyvirus resistance conditioned by recessive alleles at Bc-3.
Initiation of mRNA translation in bacteria: structural and dynamic aspects
- BiologyCellular and Molecular Life Sciences
Structural, mechanistic and dynamical aspects of translation initiation of mRNA translation are reviewed, including the first EF-G-dependent translocation marks the beginning of the elongation phase of translation.
Small Interfering RNA – Mediated Translation Repression Alters Ribosome Sensitivity to Inhibition by Cycloheximide in Chlamydomonas reinhardtii
The results suggest that sRNA-mediated repression of protein synthesis in C. reinhardtii may involve alterations to the function/structural conformation of translating ribosomes, suggesting inhibition at a postinitiation step of translation.
The Eukaryotic Translation Initiation Factor 4E Controls Lettuce Susceptibility to the Potyvirus Lettuce mosaic virus 1
The eIF4E and eIF(iso)4E cDNAs from several genotypes of lettuce ( Lactuca sativa ) that are susceptible, tolerant, or resistant to infection by Lettuce mosaic virus (LMV; genus Potyvirus ) were…
Crystal structures of GCN 2 C-terminal domains suggest regulatory differences in yeast and mammals
- Materials Science
*Address correspondence to: Millie M. Georgiadis Department of Biochemistry and Molecular Biology, 635 Barnhill Drive, Indiana University School of Medicine, Indianapolis, Indiana 46202; USA. Tel.:…
Faculty Opinions recommendation of Structural basis for autoinhibition and mutational activation of eukaryotic initiation factor 2alpha protein kinase GCN2.
Long-Term Sensitization of a Defensive Withdrawal Reflex in Aplysia
- Biology, PsychologyScience
When a weak tactile stimulus is applied to the siphon of Aplysia californica, the animal withdraws the siphons between the parapodia if the animal is previously given 4 days of training, consisting of four brief noxious stimuli each day.