Evolution of the Cannabinoid and Terpene Content during the Growth of Cannabis sativa Plants from Different Chemotypes.
@article{AizpuruaOlaizola2016EvolutionOT,
title={Evolution of the Cannabinoid and Terpene Content during the Growth of Cannabis sativa Plants from Different Chemotypes.},
author={Oier Aizpurua‐Olaizola and Umut Mehmet Ali Soydaner and Ekin {\"O}zt{\"u}rk and Daniele Schibano and Yilmaz Simsir and Patricia Navarro and Nestor Etxebarria and A. Usobiaga},
journal={Journal of natural products},
year={2016},
volume={79 2},
pages={
324-31
}
}The evolution of major cannabinoids and terpenes during the growth of Cannabis sativa plants was studied. [] Key Method Fifty clones of each mother plant were grown indoors under controlled conditions. Every week, three plants from each variety were cut and dried, and the leaves and flowers were analyzed separately. Eight major cannabinoids were analyzed via HPLC-DAD, and 28 terpenes were quantified using GC-FID and verified via GC-MS. The chemotypes of the plants, as defined by the tetrahydrocannabinolic…
231 Citations
New Methods for the Comprehensive Analysis of Bioactive Compounds in Cannabis sativa L. (hemp)
- Medicine, ChemistryMolecules
- 2018
The study on Cannabis volatile compounds was performed by developing a new method based on headspace solid-phase microextraction coupled with GC-MS and GC-FID, which was found to be the most abundant cannabinoids in the hemp samples analysed, while β-myrcene and β-caryophyllene were the major terpenes.
Terpene synthases from Cannabis sativa
- BiologyPloS one
- 2017
Functional characterization identified mono- and sesqui-TPS, whose products collectively comprise most of the terpenes of ‘Finola’ resin, including major compounds such as β-myrcene, (E)-β-ocimene, (-)-limonene, (+)-α-pinene, β-caryophyllene, and α-humulene.
(-)-trans-Δ9-Tetrahydrocannabiphorol Content of Cannabis sativa Inflorescence from Various Chemotypes.
- Biology, MedicineJournal of natural products
- 2021
The preliminary results obtained indicate Δ9-THCP (1) may have a higher degree of prevalence in C. sativa inflorescence than previously estimated.
Identification of Chemotypic Markers in Three Chemotype Categories of Cannabis Using Secondary Metabolites Profiled in Inflorescences, Leaves, Stem Bark, and Roots
- BiologyFrontiers in Plant Science
- 2021
This study investigated the utility of the full spectrum of secondary metabolites in different plant parts in three cannabis chemotypes (THC dominant, intermediate, and CBD dominant) for chemotaxonomic discrimination and provides a comprehensive profile of bioactive compounds in three chemotypes for medical purposes.
Determination of Acid and Neutral Cannabinoids in Extracts of Different Strains of Cannabis sativa Using GC-FID.
- Chemistry, MedicinePlanta medica
- 2018
A GC-FID method has been developed and validated for the qualitative and quantitative analysis of acid and neutral cannabinoids in C. sativa extracts and indicated a linear relationship between the concentration and peak area ratio with R2 > 0.999 for all 10 cannabinoids.
Terpenoids and Phytocannabinoids Co-Produced in Cannabis Sativa Strains Show Specific Interaction for Cell Cytotoxic Activity
- BiologyMolecules
- 2019
It is found that in “ high THC” or “high CBD” strains, phytocannabinoids are produced alongside certain sets of terpenoids, and the partial specificity in interaction may have influenced the cultivation of cannabis and may have implications for therapeutic treatments.
Metabolomic Analysis of Cannabinoid and Essential Oil Profiles in Different Hemp (Cannabis sativa L.) Phenotypes
- MedicinePlants
- 2021
Principal component analysis (PCA) and analysis of variance (ANOVA) showed that according to the components of essential oil, FS and TS plants were more uniform than CS plants, where there were great differences between CI and CII phenotypes.
The evolving science of phytocannabinoids
- Biology
- 2018
This Review examines phytocannabinoids in terms of their mode of action, biosynthesis, and various total syntheses and derivatizations, and describes the policy issues surrounding the possession, use and control of phytcannabinoids.
Developmental Plasticity of the Major Alkyl Cannabinoid Chemotypes in a Diverse Cannabis Genetic Resource Collection
- BiologyFront. Plant Sci.
- 2018
The results suggest that more extensive chemical and genetic characterization of the Cannabis genepool could facilitate the metabolic engineering of alkyl cannabinoid chemotypes, indicating a low level of plasticity in cannabinoid composition.
References
SHOWING 1-10 OF 58 REFERENCES
Time course of cannabinoid accumulation and chemotype development during the growth of Cannabis sativa L
- BiologyEuphytica
- 2007
It was confirmed that callus cultures of Cannabis were not able to produce detectable amounts of any cannabinoids, irrespective of the type and amount of cannabinoids in the mother plants.
Evolution of the Content of THC and Other Major Cannabinoids in Drug‐Type Cannabis Cuttings and Seedlings During Growth of Plants *
- Biology, MedicineJournal of forensic sciences
- 2012
It is not necessary to wait for plants’ flowering to identify drug‐type cannabis generally cultivated in Europe, through analysis of eight major cannabinoids content during growth.
The inheritance of chemical phenotype in Cannabis sativa L.
- BiologyGenetics
- 2003
Four crosses were made between inbred Cannabis sativa plants with pure cannabidiol (CBD) and pure Delta-9-tetrahydrocannabinol (THC) chemotypes, and it is suggested that codominance is due to the codification by the two alleles for different isoforms of the same synthase, having different specificity for the conversion of the common precursor cannabigerol into CBD or THC.
Metabolic fingerprinting of Cannabis sativa L., cannabinoids and terpenoids for chemotaxonomic and drug standardization purposes.
- Chemistry, MedicinePhytochemistry
- 2010
The inheritance of chemical phenotype in Cannabis sativa L. (II): Cannabigerol predominant plants
- BiologyEuphytica
- 2005
B0 is considered as a member of the BD allelic series encoding a CBD synthase isoform with greatly weakened substrate affinity and/or catalytic capacity, and is plausible that this B0 is a mutation of normally functional BD.
Qualitative and Quantitative Aspects of the Inheritance of Chemical Phenotype in Cannabis
- Biology
- 2003
A segregation analysis of the different F2 progenies obtained showed that chemotype, estimated as CBD/THC ratio, behaves as a qualitative character, and a model for a single locus B, with two co-dominant alleles, B D and B T is described.
Non-psychotropic plant cannabinoids: new therapeutic opportunities from an ancient herb.
- Biology, MedicineTrends in pharmacological sciences
- 2009
Taming THC: potential cannabis synergy and phytocannabinoid‐terpenoid entourage effects
- BiologyBritish journal of pharmacology
- 2011
Particular focus will be placed on phytocannabinoid‐terpenoid interactions that could produce synergy with respect to treatment of pain, inflammation, depression, anxiety, addiction, epilepsy, cancer, fungal and bacterial infections (including methicillin‐resistant Staphylococcus aureus).
The Gene Controlling Marijuana Psychoactivity
- BiologyJournal of Biological Chemistry
- 2004
It is concluded that the FAD binding residue is His-114 and that the THCA synthase reaction is FAD-dependent, the first report on molecular characterization of an enzyme specific to cannabinoid biosynthesis.






