Evolution of cell-surface acid phosphatase of Burkholderia pseudomallei.

  title={Evolution of cell-surface acid phosphatase of Burkholderia pseudomallei.},
  author={Eiji Kondo and T. Kurata and Pimjai Naigowit and Kazuo Kanai},
  journal={The Southeast Asian journal of tropical medicine and public health},
  volume={27 3},
Acid phosphatase active fractions were obtained from cell-free extract, outermembrane fraction and culture filtrate of Burkholderia pseudomallei by column chromatography with sepharose 6B and DEAE cellulose. The comparison of the elution patterns of protein, sugar and enzymatic activity among these three components suggested that the enzyme is a glycoprotein evolving from premature proteins through glycosylation and that the enzyme is translocated during glycosylation from the cytoplasm to the… CONTINUE READING

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