Evolution of a multiple antibiotic resistance region in IncHI1 plasmids: reshaping resistance regions in situ.

@article{Cain2012EvolutionOA,
  title={Evolution of a multiple antibiotic resistance region in IncHI1 plasmids: reshaping resistance regions in situ.},
  author={Amy K. Cain and Ruth M Hall},
  journal={The Journal of antimicrobial chemotherapy},
  year={2012},
  volume={67 12},
  pages={2848-53}
}
OBJECTIVES To determine the structure of the resistance region in an IncHI1 plasmid conferring resistance to multiple antibiotics, including gentamicin, recovered from a Salmonella enterica serovar Typhimurium isolate from a horse. METHODS Plasmids were recovered by conjugation. The plasmid type, resistance genes and their context were identified by PCR, cloning, hybridization and DNA sequencing. The sequence was compared using bioinformatic tools with available resistance region sequences… CONTINUE READING