Evolution of a mass spectrometry-grade protease with PTM-directed specificity.

@article{Tran2016EvolutionOA,
  title={Evolution of a mass spectrometry-grade protease with PTM-directed specificity.},
  author={Duc Trong Tran and Valerie Cavett and Vuong Q Dang and H{\'e}ctor Ricardo Lara Torres and Brian M Paegel},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2016},
  volume={113 51},
  pages={
          14686-14691
        }
}
Mapping posttranslational modifications (PTMs), which diversely modulate biological functions, represents a significant analytical challenge. The centerpiece technology for PTM site identification, mass spectrometry (MS), requires proteolytic cleavage in the vicinity of a PTM to yield peptides for sequencing. This requirement catalyzed our efforts to evolve MS-grade mutant PTM-directed proteases. Citrulline, a PTM implicated in epigenetic and immunological function, made an ideal first target… CONTINUE READING
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