Evidencing the role of lactose permease in IPTG uptake by Escherichia coli in fed-batch high cell density cultures.

The lac-operon and its components have been studied for decades and it is widely used as one of the common systems for recombinant protein production in Escherichia coli. However, the role of the lactose permease, encoded by the lacY gene, when using the gratuitous inducer IPTG for the overexpression of heterologous proteins, is still a matter of discussion… (More)