Evidence that the two binding sites for trypsin on chicken ovoinhibitor are not equivalent. Dissociation of the complexes with porcine trypsin.

Complexes between chicken ovoinhibitor (01) and porcine trypsin were allowed to dissociate in the presence of the active site titrant, j-nitrophenyl @‘-guanidinobenzoate, which reacts extremely rapidly with free active trypsin (T). The rate of the slower, “postburst” reaction with titrant, which is a measure of the rate of dissociation of the complexes… (More)