Evidence that the pathway of transferrin receptor mRNA degradation involves an endonucleolytic cleavage within the 3′ UTR and does not involve poly(A) tail shortening.

  title={Evidence that the pathway of transferrin receptor mRNA degradation involves an endonucleolytic cleavage within the 3′ UTR and does not involve poly(A) tail shortening.},
  author={Roberta Binder and J A Horowitz and James P. Basilion and David M. Koeller and Richard D. Klausner and Joe B Harford},
  journal={The EMBO Journal},
The stability of transferrin receptor (TfR) mRNA is regulated by iron availability. When a human plasma‐cytoma cell line (ARH‐77) is treated with an iron source (hemin), the TfR mRNA is destabilized and a shorter TfR RNA appears. A similar phenomenon is also observed in mouse fibroblasts expressing a previously characterized iron‐regulated human TfR mRNA (TRS‐1). In contrast, mouse cells expressing a constitutively unstable human TfR mRNA (TRS‐4) display the shorter RNA irrespective of iron… 
Characterization of the Translation-dependent Step during Iron-regulated Decay of Transferrin Receptor mRNA*
Observations suggest that inhibition of translation by cycloheximide interferes with the rate-limiting step of iron-induced TfR mRNA decay in a trans-acting mechanism by blocking IRP inactivation.
β-Globin mRNA decay in erythroid cells: UG site-preferred endonucleolytic cleavage that is augmented by a premature termination codon
The data suggest that an endonuclease with preference for UG dinucleotides is involved in the degradation of nonsense-containing and, to a lesser extent, nonsense-free human β-globin mRNAs in mouse erythroid cells.
The Poly(A)-Binding Protein and an mRNA Stability Protein Jointly Regulate an Endoribonuclease Activity
It is demonstrated that ErEN activity is regulated by the poly(A) tail, and PABP was shown to enhance the binding efficiency of αCP to the α-globin 3′UTR, which in turn protected the ErEN target sequence.
Degradation of the unstable EP1 mRNA in Trypanosoma brucei involves initial destruction of the 3'-untranslated region.
The degradation of three reporter mRNAs in Trypanosoma brucei is described, including the 3'-untranslated region (3'-UTR) from the developmentally regulated EP1 mRNA, which is abundant in the procyclic form of the parasite but is almost undetectable in the bloodstream form.
The cis-acting elements involved in endonucleolytic cleavage of the 3' UTR of human IGF-II mRNAs bind a 50 kDa protein.
In vivo cross-linking data show that a cytoplasmic protein with an apparent molecular weight of 48-50 kDa, IGF-II cleavage unit binding protein (ICU-BP), that binds to the stem structure formed by interaction of parts of the cis-acting elements I and II.
A polysomal ribonuclease involved in the destabilization of albumin mRNA is a novel member of the peroxidase gene family.
Cloning of an approximately 60 kDa endoribonuclease from Xenopus liver polysomes with properties expected for a messenger RNase involved in the estrogen-regulated destabilization of serum protein mRNAs is described and its identification as a novel member of the peroxidase gene family is described.
Effect of Transcription Inhibitors on the Iron-dependent Degradation of Transferrin Receptor mRNA *
It is demonstrated that part of the active IRP co-localizes with TfR mRNA to the rough endoplasmic reticulum, indicating that IRP dissociates prior to T fR mRNA decay.
Regulation of the Stability of Heat-Stable Antigen mRNA by Interplay between Two Novel cis Elements in the 3′ Untranslated Region
It is shown that a 160-bp element, located in the region of nucleotides 1465 to 1625 in the 3′UTR of HSA mRNA, promotes RNA degradation and this effect is neutralized by a 43-bp fragment approximately 1 kb upstream of the negative cis element.
Destabilization of Human α-Globin mRNA by Translation Anti-termination Is Controlled during Erythroid Differentiation and Is Paralleled by Phased Shortening of the Poly(A) Tail*
A characteristic organization of the poly(A) tail on α-globin mRNA which is maintained during normal and accelerated decay, a correlation between poly (A) metabolism and anti-termination-mediated accelerated mRNA turnover, and a switch in the mechanism of mRNA decay during erythroid terminal differentiation are demonstrated.
Identification of in Vivo mRNA Decay Intermediates Corresponding to Sites of in Vitro Cleavage by Polysomal Ribonuclease 1*
This study sought to determine whether the in vivo destabilization of albumin mRNA following estrogen administration involves the generation of decay intermediates that could be identified as products of PMR-1 cleavage.


Control of messenger RNA stability.
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The content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories.
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